Pentecost, also known as Whit Sunday or Whitsunday, is celebrated annually by Christians originating from the Jewish holiday of Shavuot. In Vatican City, one of the centers of Christianity, it is highly significant and is observed with deep reverence. On this day, believers commemorate the descent of the Holy Spirit upon the Apostles and other followers of Jesus Christ as described in the Acts of the Apostles 2:1–31. This event, occurring fifty days after Easter Sunday, marks the birth of the Christian Church.

In Vatican City, the spirit of this holiday is embraced wholeheartedly as it is an important liturgical feast in the Church's calendar. Notably, the Pope celebrates a Mass in St. Peter's Square on this day to mark the event, which is attended by thousands of pilgrims from around the world. People flock to Vatican City to participate in this grand celebration, where the Pope traditionally serves as the principal celebrant. The descent of the Holy Spirit on the apostles is portrayed through the symbol of a dove, which also signifies peace. This day is a reminder of the unity of the Church and the gift of the Holy Spirit's ongoing work in the world today.

For residents and visitors in Vatican City, the day of Pentecost is a moment of communal gathering, prayer, and sharing of faith. It is a public holiday in the Vatican City and life quietens down as shops close and locals as well as visitors spend their time attending religious ceremonies. The atmosphere is often filled with a sense of tranquility and deep devotion. This holiday serves as a reminder for Christians of their religious foundation and rekindles their spiritual commitment.